Tickpedia
Removal · field guidance

CDC two-tier serology for Lyme

Standard Lyme workup: a sensitive ELISA or IFA first, then a Western blot only if the first test is positive or equivocal.

Steps

schema.org/HowTo
  1. 01
    Order a first-tier EIA/ELISA or IFA against Borrelia burgdorferi antigens.
  2. 02
    If negative and symptoms have lasted under 30 days, consider repeating in 4-6 weeks.
  3. 03
    If positive or equivocal, reflex to a supplemental Western blot.
  4. 04
    Run IgM and IgG blots if symptoms < 30 days; IgG only if symptoms > 30 days.
  5. 05
    Interpret per CDC band criteria; report a positive only when both tiers are positive.

Wild facts

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